Question

what is RT-PCR test?

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Answer 1

Explanation:

Shimla: Relaxing the existing COVID-19 restrictions, the Himachal Pradesh Cabinet, on Friday, decided to do away with the requirement of RT-PCR tests for entering the state. A decision was also taken to increase the shop opening timings from 9 am to 5 pm from June 14.

Shops have, however, been asked to remain closed on weekends. According to the latest guidelines issued by the state governments, offices can function with 50 per cent of staff. Further, intra-state public transport has been allowed to operate with 50 per cent occupancy, thus allowing easy commuting options to the local population.

Answer 2

Answer:

here is full defination

Explanation:

Real-time PCR has become one of the most widely used methods of gene quantitation because it has a large dynamic range, boasts tremendous sensitivity, can be highly sequence-specific, has little to no post-amplification processing, and is amenable to increasing sample throughput. However, optimal benefit from these advantages requires a clear understanding of the many options available for running a real-time PCR experiment. Starting with the theory behind real-time PCR, this review discusses the key components of a real-time PCR experiment, including one-step or two-step PCR, absolute versus relative quantitation, mathematical models available for relative quantitation and amplification efficiency calculations, types of normalization or data correction, and detection chemistries. In addition, the many causes of variation as well as methods to calculate intra- and inter-assay variation are addressed.

Introduction

The advent of real-time PCR and real-time reverse transcription PCR (real-time RT-PCR) has dramatically changed the field of measuring gene expression. Real-time PCR is the technique of collecting data throughout the PCR process as it occurs, thus combining amplification and detection into a single step. This is achieved using a variety of different fluorescent chemistries that correlate PCR product concentration to fluorescence intensity (1). Reactions are characterized by the point in time (or PCR cycle) where the target amplification is first detected. This value is usually referred to as cycle threshold (Ct), the time at which fluorescence intensity is greater than background fluorescence. Consequently, the greater the quantity of target DNA in the starting material, the faster a significant increase in fluorescent signal will appear, yielding a lower Ct (2

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