what is sensitivity stain
Answers
Answer:
संवेदनशीलता धर्म का आधार होता है - दया, करुणा और अनुकम्पा। इसके लिए मानव में मानवीयता का गुण होना आवश्यक है। ... आज जितनी भी आपराधिक घटनाएँ होती है, उसका मूल कारण संवेदनशीलता का अभाव है जिसकी वज़ह से मनुष्य दूसरों के दुःख, दर्द और पीड़ा का अनुभव नहीं कर पाता।
Answer:
To study the sensitivity and specificity of different staining methods to monitor apoptosis induced by oxidative stress in adherent cells.
Methods: Sensitivity and specificity of several common methods for apoptosis determination were evaluated (Apo2.7-expression, Annexin V-binding, TUNEL-reaction, poly-(ADP-ribose)-polymerase-(PARP) cleavage and single-stranded-DNA (ssDNA) staining). Apoptosis was induced by oxidative stress generated by hydrogen peroxide in 3 cultured cells types growing as adherent monolayer (MiaPaCa-2, Hep-G2 and human skin fibroblasts), necrosis was induced by depletion of cellular ATP using sodium azide. Cells positively stained by the respective apoptosis assay were quantified and alterations of cell morphology were monitored by fluorescence microscopy. The date was analyzed by one-way analysis of variance and significance test of correlation coefficient.