What is the effect of adding tryptophan to a culture of e. coli?
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High tryptophan: The tryptophan binds to the trp repressor and causes it to change shape, converting into its active (DNA-binding) form. The trp repressor with the bound tryptophan attaches to the operator, blocking RNA polymerase from binding to the promoter and preventing transcription of the operon.
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High tryptophan: The tryptophan binds to the trp repressor and causes it to change shape, converting into its active (DNA-binding) form. The trp repressor with the bound tryptophan attaches to the operator, blocking RNA polymerase from binding to the promoter and preventing transcription of the operon.
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