Question

What is the effect of chloramphenicol and cycloheximide on saccharomyces cerevisiae?

Answer 1

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Answer 2

The action of two inhibitors of protein synthesis, chloramphenicol and cycloheximide, on the production of lipids during chloroplast formation in the unicellular alga Euglena gracilis has been investigated. Under conditions where chloramphenicol prevented chlorophyll and chloroplast protein formation, the production of the galactolipids, monogalactosyl diacylglycerol, and digalactosyl diacylglycerol was also inhibited, although phospholipid synthesis was not affected. At low concentrations of cycloheximide (2.5 μg/5 × 106 cells), cell division was completely inhibited, but after an initial lag period of about 12 hr, chlorophyll production began at an accelerated rate. After 72 hr illumination, such cells contained substantially more chlorophyll than control cultures. Higher concentrations of cycloheximide increased the lag period before chlorophyll production began. Phospholipid production was severely inhibited in cycloheximide-treated cells, but the production of galactolipids showed the same pattern as that of chlorophyll, a lag phase followed by a period of accelerated synthesis. Cycloheximide also affected the molar ratio of monogalactosyl diacylglycerol to digalactosyl diacylglycerol, and the fatty acid composition of the galactolipids. The galactolipids of cycloheximide-treated cells contained increased amounts of polyunsaturated fatty acids containing 16 or 18 carbon atoms.