What is the functional significance of glacial acetic acid present in Nippe’s reagent.
Answers
Answer:
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Explanation:
The haemin crystals are prepared by heating of the blood with Nippe’s
reagent. This ruptures the RBCs and the haemoglobin is released. Also, in this
process the ferrous form of iron is converted to ferric form. The globin protein
gets denatured by heating with acetic acid; while, heme is converted to
oxidized heme called haematin. The haematin combines with halogens such
as chloride ions to form insoluble haemin which appear as rhombic crystal of
chocolate brown colour. The haemin crystals or hydrochloride of heme are characteristic of blood and are thus, used for identification of blood
stains
question given →What is the functional significance of glacial acetic acid present in Nippe’s reagent.
answer→ Haemin crystals are used in medico-legal practices to distinguish fresh or dried blood stains from other red-coloured stains. The shape of haemin crystals varies in different species and thus, blood stains of human can be confirmed.