What is the principle of zonal centrifugation?
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The centrifuge works using the sedimentation principle, where the centrifugal acceleration causes denser substances and particles to move outward in the radial direction. At the same time, objects that are less dense are displaced and move to the center.
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In rate-zonal centrifugation, the sample to be analysed is layered on a preformed density gradient and subjected to centrifugal force for a defined length of time. The particles sediment through the gradient at a rate that is a function of their sedimentation coefficient.
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