Question

What is the purpose of insertional inactivation if all recombinants die and non recombinants survive; do we need recombinants or non recombinants?

Answer 1

we need is recombinants not the non recombinants
due to insertion inactivation recombs die we can only identify which one is the recombinant from that ,nothing more

Answer 2

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Insertional inactivation is a technique used in recombinant DNA technology. In this procedure, a bacteria carrying recombinant plasmids or a fragment of foreign DNA is made to insert into a restriction site inside a gene to resist antibiotics, hence causing the gene to turn non-functional or in an inactivated state.