What is the relevence of tissue fixation in histological processing?
Answers
Answer:
Tissue Dehydration. .....
Dehydration is simply the removal of water from aqueous-fixed tissue. Since most fixatives are aqueous, this step is necessary to prepare the tissue for embedding in non-aqueous media like paraffin.
Hope it's help✌☺❤gm
Answer:
Tissue Fixation
fixation of it tissue sample is essential to maintain cell and tissue morphology during the IHC experiment and during storage.
It also prevents the autolysis and necrosis of excited tissue, preserves antigenecity and enhances the refractive indexof tissues constituents.
The fixative used is influenced by the target antigen as well as desired dectation technique .
Embedding is important in preserving tissue morphology and giving tissue support during sectioning.
The Tissue is typically cut into thin sections aur smaller pieces (for whole mount studies) to facilities further studies.
Thanks.........
I hope it is helpful for all...........
Please mark me as a brain list...........