what is the result of chrametography experiment?
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The purpose of this experiment is to observe how chromatography can be used to separate mixtures of chemical substances. ... It is used for the purification and isolation of various substances. A chromatographically pure substance is the result of the separation.
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Chromatography is one of the most powerful tools in analytical chemistry and is one of the most commonly found instruments in the laboratory. In this technique, the mobile phase is a liquid.
High-Performance Liquid Chromatography (HPLC)
High-pressure liquid chromatography, now known as high-performance liquid chromatography (HPLC), is a chromatographic technique used to identify, quantify, separate and purify individual compounds present in a mixture [1].
How does high-performance liquid chromatography work?
In high-performance liquid chromatography (HPLC) the sample mixture is passed along with a liquid solvent under high pressure through a column filled with a solid adsorbent material. The pressure within the system is built-up with the help of pumps. The working principle is that each compound in the mixture interacts slightly different with the adsorbent material in the column, resulting in varying flow rates for the different components. This leads to separation of the components as they flow out of the column. The adsorbent material used is typically granular, made up of solid particles, such as silica, constituting the ‘stationary phase’. The pressurized liquid is a mixture of solvents, such as water and organic liquids like methanol and acetonitrile, which constitute the ‘mobile phase’.
The detector is connected to a digital microprocessor and user software for data acquisition and analysis. The separated compounds are visualized as peaks with the number of peaks corresponding to the number of separated components in the mixture. The area of the peak is proportional to the concentration of the compound present within the mixture [2]. The resolution between two peaks in chromatographic techniques is the extent to which substances are separated during the experiment. Higher resolution reflects good separation of the compounds.
What is high-performance liquid chromatography used for?
High-performance liquid chromatography has a wide range of applications in the fields of biochemistry, analytical chemistry, pharmaceuticals, forensics, food research, and other areas. For example, HPLC is also used in the testing for banned substances in athletes.
Why would you use high-performance liquid chromatography?
HPLC is both affordable and adaptable. It provides useful benefits such as data management and instrument validation.
Ultra High-Performance Liquid Chromatography (UHPLC)
Ultra-high performance liquid chromatography (UHPLC) is a variant of HPLC which uses smaller particles to enhance chromatographic performance and requires higher pressures. In a similar way to HPLC, the water contaminants can negatively impact on results, however, due to the higher sensitivities of the UHPLC, this can be more extreme.
Both HPLC and UHPLC can be used for liquid chromatography, but the equipment needed to run both differs and each has different benefits. The shorter column length means that Ultra high-performance liquid chromatography provides a better resolution than traditional HPLC.
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High-Performance Liquid Chromatography (HPLC)
High-pressure liquid chromatography, now known as high-performance liquid chromatography (HPLC), is a chromatographic technique used to identify, quantify, separate and purify individual compounds present in a mixture [1].
How does high-performance liquid chromatography work?
In high-performance liquid chromatography (HPLC) the sample mixture is passed along with a liquid solvent under high pressure through a column filled with a solid adsorbent material. The pressure within the system is built-up with the help of pumps. The working principle is that each compound in the mixture interacts slightly different with the adsorbent material in the column, resulting in varying flow rates for the different components. This leads to separation of the components as they flow out of the column. The adsorbent material used is typically granular, made up of solid particles, such as silica, constituting the ‘stationary phase’. The pressurized liquid is a mixture of solvents, such as water and organic liquids like methanol and acetonitrile, which constitute the ‘mobile phase’.
The detector is connected to a digital microprocessor and user software for data acquisition and analysis. The separated compounds are visualized as peaks with the number of peaks corresponding to the number of separated components in the mixture. The area of the peak is proportional to the concentration of the compound present within the mixture [2]. The resolution between two peaks in chromatographic techniques is the extent to which substances are separated during the experiment. Higher resolution reflects good separation of the compounds.
What is high-performance liquid chromatography used for?
High-performance liquid chromatography has a wide range of applications in the fields of biochemistry, analytical chemistry, pharmaceuticals, forensics, food research, and other areas. For example, HPLC is also used in the testing for banned substances in athletes.
Why would you use high-performance liquid chromatography?
HPLC is both affordable and adaptable. It provides useful benefits such as data management and instrument validation.
Ultra High-Performance Liquid Chromatography (UHPLC)
Ultra-high performance liquid chromatography (UHPLC) is a variant of HPLC which uses smaller particles to enhance chromatographic performance and requires higher pressures. In a similar way to HPLC, the water contaminants can negatively impact on results, however, due to the higher sensitivities of the UHPLC, this can be more extreme.
Both HPLC and UHPLC can be used for liquid chromatography, but the equipment needed to run both differs and each has different benefits. The shorter column length means that Ultra high-performance liquid chromatography provides a better resolution than traditional HPLC.
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