what is the role of colchicine in karyotype?
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In routine karyotyping procedure from lymphocyte culture (blood culture) cells are exposed to colchicine to arrest cell division at metaphase to visualise chromosomes. ... To get better quality of chromosomes both the concentration and period of exposure to colchicine can be altered.
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Colchicine is a type of plant alkaloid and a kind of mitotic inhibitors used in karyotyping. As we said, it prevents the formation of spindle fibers or microtubules to arrest cell at metaphase. ... It goes into our cell culture and arrests all cells to metaphase.
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