What is the role of dishwasher in the separation of dna from vegetable mass using household items
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There are three basic and two optional steps in a DNA extraction: Cells which are to be studied need to be collected. Breaking the cell membranes open to expose the DNA along with the cytoplasm within (cell lysis). Lipids from the cell membrane and the nucleus are broken down with detergents and surfactants.
The role of the salt is to neutralize the charge of the DNA's sugar phosphate backbone. ... Ethanol has a lower dielectric constant than water so it's used to promote ionic bonds between the Na+ (from the salt) and the PO3- (from the DNA backbone) causing the DNA to precipitate.
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