What is the role of EDTA in DNA extraction?
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EDTA helps to stop DNases from acting on the exposed DNA, which is obviously important if you’re trying to get the DNA for a specific purpose, like sequencing. DNases are enzymes that “chew up” DNA, so it’s important to keep them from acting on your DNA of interest.
EDTA is responsible for chelation of divalent ions. It stops the action of DNases found in cytoplasm of cells.
For DNA extraction, cells and nucleus need to be disrupted. Therefore, DNA comes in contact with DNases present in the cytoplasm. These DNases, DNA cutting enzymes, can destroy the genomic DNA and reduce the yield of gDNA considerably. Mg2+ is an important factor for activity of DNases. EDTA deprives the enzyme of this co-factor and renders it inactive.
EDTA is responsible for chelation of divalent ions. It stops the action of DNases found in cytoplasm of cells.
For DNA extraction, cells and nucleus need to be disrupted. Therefore, DNA comes in contact with DNases present in the cytoplasm. These DNases, DNA cutting enzymes, can destroy the genomic DNA and reduce the yield of gDNA considerably. Mg2+ is an important factor for activity of DNases. EDTA deprives the enzyme of this co-factor and renders it inactive.
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