What is the role of heat treatment at 80 in glucose estimation from blood serum by folin wu method?
Answers
in folin wu's method,in second step,we add molybic acid and sodium tungstate with sodium hydroxide and water in order to form phosphomolybdic acid.we heat the whole mixture so formed vigorously to remove ammonia present in the molybic acid.if it is not removed then it can form its complex with copper sulphate (alkaline) .due to this it will become unavailable to react with the given sample of deprotinised blood.and we cant proceed the glucose estimation further.
Folin Wu method
Explanation:
In Folin Wu method of glucose estimation, we are required to add molybic acid and sodium tungstate with sodium hydroxide and water in order to form phosphomolybdic acid. In this step, ammonia is present and it needs to be removed. Otherwise, it will form a complex with copper sulphate and it won't react with the given sample of deprotinised blood. This will make us unable to proceed with glucose the estimation. Hence heat treatment becomes essential at the right stage.