Question

What is the role of janus green b stain in this slide preparation

Answer 1

Answer:

Explanation:

Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900.[2]

Janus Green B

[1]

Janus green B.svg

Ball-and-stick model of the component ions of Janus Green B

Janus Green B powder.jpg

Solid Janus Green B

Janus Green B solution.jpg

Janus Green B Solution

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Answer 2

Answer:

The role of Janus green b stain in slide preparation is discussed below.

EXPLANATION:

• Janus green B is a basic dye and a vital stain.

• The vital stain is prepared by dissolving from 0.001 to 0.41 g in 100 ml of physiological saline solution.

• It used in various high dilutions as a vital stain for flagella of the protozoa and the fungi.

• Get a piece of celery and prepare it in slide, observe it under a microscope.

• pour some drops of Janus green b and place the paper towel on the other slide of the coverslip.

• The stain will be drawn into mitochondria and turn them greenish-blue.