Chemistry, asked by prathameshc3963, 9 months ago

What is the role of lysis solution 1in plasmid isolation?

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Answered by Anonymous
0

Alkaline lysis is a method used in molecular biology, to isolate plasmid DNA or other cell components such as proteins by breaking the cells open. ... The detergent cleaves the phospholipid bilayer of membrane and the alkali denatures the proteins which are involved in maintaining the structure of the cell membrane.

Answered by XxItzZalimGudiyaxX
1

Answer:

Bacteria are lysed with a lysis buffer solution containing sodium dodecyl sulfate (SDS) and sodium hydroxide. During this step disruption of most cells is done, chromosomal as well as plasmid DNA are denatured and the resulting lysate is cleared by centrifugation, filtration or magnetic clearing.

By XxItzZalimGudiyaxX

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