Question

What is the use of mgcl2 in avoiding dimerization in pcr?

Answer 1

he basics of Magnesiums function in PCR. MgCl2 acts as a cofactor and is a catalyzer in PCR. That means, higher concentrations of MgCl2 increases higher productivity of Taq polymerase. But the specificity will be less with high productivity and causes ugly band smears in your gel.

Answer 2

your answer

its higher productivity will increase the productivity of TAQ POLYMERASE but this would lead to less specifity