Question

What process we should follow to observe lactobacillus bacteria in the lab

Answer 1

Answer:

Materials required: Curd or yogurt (or any other source of lactobacillus), glass slide, cover slip, tooth pick, methylene blue stain, tissue paper, light microscope.

Procedure:

1. Put a drop of curd on a clean test tube with the help of tooth pick.
2. Smear it with the help of another slide.
3. Put a drop of methylene blue on the smear and leave it for few seconds.
4. Put the cover slip on the slide where smear is uniformly spread.
5. One can remove excess stain with the help of tissue or paper towel.
6. Observe the slide under light microscope; first under low power then in high power if needed.

Observation: They will appear as purple or blue colored rod shaped organisms.

Answer 2

ANSWER :

1) Take one or two drops of buttermilk on a slide , spread it on the slide .

2) Heat it slightly on a lamp for 3-4 seconds.

3) Add a few drops of crystal violet on it .

4) After 30 to 60 seconds , wash the slide gently with water .

5) Observe the slide under the compound microscope .

6) We will find a stick like structures , known as bacteria .

HOPE ITS HELPFUL