Question

What radioactive molecule is used to end-label an oligonucleotide?
A.(alpha)-32P-ATP
B. (alpha)-32P-dUTP
C. (gamma)-32P-ATP
D. Any of these

Answer 1

b is the answer .hope this answer helpful for you

Answer 2

Answer:

B. (alpha)-32P-dUTP

Explanation:

Many experimental techniques for figuring out nucleic acid feature require labeling the nucleic acid with radioisotopes or a chemical tag. Labels allow nucleic acid detection, yield records approximately its state, and may function a deal with through which the nucleic acid and related elements may be purified from a mixture. Radioactive phosphate is generally introduced to the 5′ or 3′ cease of an oligonucleotide post-synthesis the usage of enzyme-catalyzed reactions. In contrast, chemical tags are commonly introduced all through synthesis or the usage of reactive corporations which might be included all through synthesis. Here, we gift protocols for post-artificial conjugation of chemical tags to unmodified RNA or DNA oligonucleotides.

The technique may be used to connect fluorescent dyes and biotin corporations to oligonucleotides, and to immobilize oligonucleotides to a strong support. Oligonucleotides tagged with fluorescent dyes are quite simply detected in each gel- and plate reader-primarily based totally assays, at the same time as biotin or resin conjugated oligonucleotides are beneficial equipment for affinity purification. Fluorescent cease-labeling affords numerous benefits over radioactive labeling, lowering radioactivity-related risks and yielding a classified molecule that doesn't decay, at the same time as presenting the sensitivity required for plenty procedures.

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