Question

What s non ideality of chromatogram in chromatography is due to?

Answer 1

An ideal chromatography peak is a nice sharp symmetrical shape, a Gaussian peak, on a flat baseline. A peak can deviate from this ideal in several different ways. It can become asymmetrical, flatten and become broader, or the baseline can rise.

One of the common shifts away from a Gaussian peak is when the back half of the peak falls away. If the peak were split into two, vertically, the later half would be wider than the first half of the peak. This effect is most clearly seen close to the baseline and is known as peak tailing.