Question

What should spotting of mixture be done above the level of mobile phase

Answer 1

Answer:

1) The spots can dissolve into the solvent if the recognizing line is blow the solvent level. As a result, they wont travel it up the plate within the mobile part.

2) because mobile has several harmful particles therefore we must always not spot whereas doing experiments with mixtures  

3) Also, some compounds that were noticed were in all probability low in concentration in order that they were invisible on the tending.

Answer 2

Answer:

In thin-layer chromatography( TLC), the sample mixture should be spotted above the solvent level. It is because if the mixture gets in contact with the solvent initially, it dissolves and gets washed off in the solvent before traveling up the plate.

Ideally, the solvent layer should rise gradually by capillary action and along with it, the sample mixture should also travel up the thin layer chromatography plate.