What to do if protein got precipitated during refolding?
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HEY MATE HERE IS YOUR ANSWER:-
We occasionally get precipitation during dialysis, I think some proteins do not like being in the presence of imidazole for long periods. One solution to avoid this problem is to use a desalting column which rapidly buffer exchanges away the imidazole.
Also 15 mg/ml is a pretty high concentration, you could try diluting it and performing the dialysis at a lower concentration, then concentrate it back down afterwards.
MARK AS BRAINLIEST! :)
We occasionally get precipitation during dialysis, I think some proteins do not like being in the presence of imidazole for long periods. One solution to avoid this problem is to use a desalting column which rapidly buffer exchanges away the imidazole.
Also 15 mg/ml is a pretty high concentration, you could try diluting it and performing the dialysis at a lower concentration, then concentrate it back down afterwards.
MARK AS BRAINLIEST! :)
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