what was two distinguished form of protoplasm and where they located
Answers
Protoplasm is a mixture of small molecules such as ions, monosaccharides, amino acid, and macromolecules such as proteins, polysaccharides, lipids, etc.
Protoplasm is the living part of a cell that is surrounded by a plasma membrane.
In some definitions, it is a general term for the cytoplasm (e.g., Mohl, 1846),[1] but for others, it also includes the nucleoplasm (e.g., Strasburger, 1882). For Sharp (1921), "According to the older usage the extra-nuclear portion of the protoplast [the entire cell, excluding the cell wall] was called "protoplasm," but the nucleus also is composed of protoplasm, or living substance in its broader sense. The current consensus is to avoid this ambiguity by employing Strasburger's [(1882)] terms cytoplasm [coined by Kölliker (1863), originally as synonym for protoplasm] and nucleoplasm ([term coined by van Beneden (1875), or] karyoplasm, [used by] Flemming [(1878)])".[2][3][4][5][6] The cytoplasm definition of Strasburger excluded the plastids (Chromatoplasm).
Like the nucleus, whether to include the vacuole in the protoplasm concept is controversial.