What will be the faith of lag phase if i increase or decrease the size of inocula?
Answers
Basically there are two kinds of lag phases, as defined by Pirt (1975).
1- A true lag which is defined as a lag caused by factors such as change in physiology, change in nutrients, presence of inhibitors, spore germination, state of inoculum culture.
2- An apparent lag, where a fraction of the inoculated cells are deviding normally while another fraction is dying or simply not deviding.
The result in both cases is that your cell count is constant. therefore, in the 2nd case, increasing your inoculum size will lead to an increase of the deviding (active) cell fraction. In other words:
growth rate is defined as:
dX/dt = µX
if only a fraction of the cells are deviding, then:
dX/dt = µX*F; where F is the fraction of the active cell population and where µ*F in the apparent growth rate.
If you increase your X (inoculum size), then you F will tend to 1 and µ*F will tend to µ.
In microbiogical processes, inoculum size can be high, especially in the case of yeasts. In industrial processes for yeast production, where performances are required, the inoculum size can represent 40% in terms of v/v. In these cases, Inoculum has to be prepared and concentrated before adding it in the reactor.
You will find more details in Principles of microbe and cell cultivation (Pirt, 1975).
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