What will be the steps to study the different parts of an embryo of pea seeds ?
Answers
Experiment to Identify Different Parts of an Embryo of a Dicot Seed!
Experiment:
Objective:
To identify the different parts of an embryo of a dicot seed (Pea, gram or red kidney bean).
Apparatus and Materials Required:
Permanent slides of dicot embryo showing different stages, and a compound microscope.
Theory:
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During sexual reproduction male gamete fuses with female gamete and zygote is formed. After some rest period zygote divides, re-divides and finally develops into an embryo.
In the earlier stages of development of embryo there is no difference between monocots and dicots, but their development differs in later stages. The process of development of mature embryo from zygote is called embryogeny.
Procedure:
1. Observe each permanent slide in sequence from early stages to maturity first under low-power magnification and then under high-power magnification of a compound microscope.
2. Draw diagrams and identify the different parts.
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3. Label the different regions of the embryo.
Observation:
1. Zygote divides transversely forming a basal cell towards the micropyle and a terminal cell towards the chalaza.
2. The basal cell divides by transverse divisions and finally forms 6-10 celled suspensors.
3. The uppermost cell of suspensor swells up to form a vesicular cell while the lowest cell of the suspensor is called hypophysis. Hypophysis forms part of the radical and root cap.
4. The terminal embryonal cell divides by transverse and vertical divisions and forms 16-celled globular embryo.
5. The globular embryo later becomes heart-shaped due to its differentiation into cotyledons.
6. The embryo and cotyledons become larger and curved.
Different Stages in the Development of Dicot Embryo
Result:
The prepared permanent slides show successive stages of the development of dicot embryo. The young dicot embryo is globular but changes to heart-shaped structure at maturity due to differentiation into cotyledons.
Precautions:
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1. Focus the slides properly.
2. Study the slides first under low-power magnification and then under high-power magnification of the compound microscope.
3. Draw diagrams of the stages as seen under the microscope.
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DIFFERENT PARTS OF A PEA SEED.
To study parts of a seed, first it's essential to collect and bring yo laboratory the seeds to be used in the case study. Lay down procedures to be used in identifying the parts of the seed in groups by the help of the teacher
Distribute the seeds in group
Each member to participate in bisecting the seed equally to view the inner components o the seeds.
Identify differnt parts as you note them down. You can name them against their functions and role in plants life. Parts include embryo, ovary, stalk and seeds that each plays its own role.
Engage the teacher in your discussion in order to seek clarification and corrections in times of disagreements.