what will happen if we place agar medium beside decapited plants
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To prepare the leaf discs around 30 ml of (1.5% agar solution was poured into a 7 cm Petri dish) after cooling the agar (simply agar) leaf in this media and condition was able to survive 10 days, all tool used in cutting the leaves were sterilized in 90% ethanol to reduce contamination. I was able to keep the leaf for at least 10 days simply. I add parafilm covering over the dishes.
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