Question

What would be the base sequence of RNA transcript obtained from the given DNA segment ?
5\' - G C A T T C G G C T A G T A A C-3` Coding strand of DNA
3\' - C G T A A G C C G A T C A T C A T T G-5\' Non - coding strand of DNA

Answer 1

Explanation:

Transcription is the first step of DNA based gene expression, wherein a segment (or whole) of a gene's DNA segment is copied (transcribed) into RNA, with the help of a group of enzymes called RNA Polymerases. RNA Polymerases use a single-stranded DNA template to synthesize a complementary strand of RNA. Specifically, RNA polymerase builds an RNA strand in the 5' to 3' direction, adding each new nucleotide to the 3' end of the strand.

Therefore, option C is incorrect.

From the above information, we can say that the coding strand (of the DNA) and the RNA sequence so transcribed are similar, but not identical (RNA would code Uracil instead of Thymine).

Thus, the correct answer would be B.

Option A shows the template strand, i.e the complementary sequence to the coding strand (given in the question). This template strand would then code the mRNA sequence.

Thus, an incorrect choice.

Option D shows the template strand with the wrong direction of coding ( the template strand would code complementary sequence from 3' → 5', as opposed to the one in the option)

Therefore, is a wrong choice.

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