When brown colour of protein bands appear during silver staining?
Answers
Answer:
After silver impregnation for 30 min, more silver is incorporated into the denatured proteins, which results in a brownish color. The uniform distribution of silver grains in brown bands and negative bands is due to silver reduction, which requires no initiation site.
Explanation:
Silver staining is the most sensitive colorimetric method for detecting total protein. The technique involves the deposition of metallic silver onto the surface of a gel at the locations of protein bands. Silver ions (from silver nitrate in the staining reagent) interact and bind with certain protein functional groups.
Answer:
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