Biology, asked by Akashshibu4674, 10 months ago

When brown colour of protein bands appear during silver staining?

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Answered by Anonymous
0

Answer:

After silver impregnation for 30 min, more silver is incorporated into the denatured proteins, which results in a brownish color. The uniform distribution of silver grains in brown bands and negative bands is due to silver reduction, which requires no initiation site.

Explanation:

Silver staining is the most sensitive colorimetric method for detecting total protein. The technique involves the deposition of metallic silver onto the surface of a gel at the locations of protein bands. Silver ions (from silver nitrate in the staining reagent) interact and bind with certain protein functional groups.

Answered by mehek2440
1

Answer:

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