When [S] = Km, the velocity of an enzyme catalyzed reaction is about?
Select one:
a. 0.1 Vmax
b. 0.9 Vmax
c. 0.4 Vmax
d. 0.7 Vmax
e. 0.5 Vmax
Answers
Answer:
How to calculate the km and Vmax values of an enzyme when I have substrate/product inhibition?
Hello,
Could anyone please help me in calculating the Km and Vmax values of an enzyme (I am working on dihydrofolate reductase DHFR) when I have substrate/product inhibition?
I see the inhibition on (specific activity nmol/min/mg to substrate concentration µM) curve, with substrate concentrations near the theoretical Vmax. (I mean the calculated Vmax by ignoring the inhibition).
(I attached a link of the curve)
- More details:
the Km of my enzyme is about 1µM. (in case I ignored the inhibition).
The specific activity with 5µM substrate is 2µM/min/mg. (in my case this is Vmax when I ignore the inhibition).
The specific activity with 10µ substrate is 1,7µM/min/mg. (it is lower here, that means here we have inhibition).
The specific activity with 20µM substrate is 1,5µM/min/mg. (again inhibition).
I don't know what is the right method to calculate the Vmax and Km for that enzyme and substrate with that inhibition phenomenon, waiting kindly for your guiding.
Explanation:
Answer: a
Explanation:
It's 80%