Which is the best way to separate intact chloroplast from green leaves of angiosperm?
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In chloroplast isolation method, the cell wall is broken mechanically using a blender or homogenizer. Then the unbroken leaf tissue and the cellular debris are removed by filtration. The chloroplasts are collected by centrifugation using a percoll gradient.
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Explanation:
Organelles from Spinach Leaf ... by sucrose density gradient centrifugation to separate constituent cell organelles. .... Aminotransferases in peroxisomes from spinach leaves.
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