Question

Which of the following enzymes’ combined action leads to the generation of sticky ends in the plasmid vector? a) Alkaline phosphatase and terminal transferase b) Exonuclease III and alkaline phosphatase c) Bacteriophage lambda exonuclease and terminal transferase d) Exonuclease III and terminal transferase

Answer 1

Answer:

In hilly areas, the soil is clayey and sticky. true or false

Answer 2

C) Bacteriophage lambda exonuclease and terminal transferase combined action leads to the generation of sticky ends in the plasmid vector.

The function of Lambda Exonuclease :

• Lambda exonuclease could be an $5'-- > 3'$exonuclease that debases double-stranded DNA (dsDNA).
• Hereditarily indistinguishable coupling proteins utilize the single-stranded DNA produced by lambda exonuclease to conduct out homologous recombination.

The function of terminal transferase (TdT) :

• The expansion of deoxynucleotides to the $3'$ hydroxyl end of DNA particles is catalysed by terminal transferase (TdT), a template-independent polymerase.
• DNA atoms with bulging, sticky, or blunt-ended double or singular strands.
• This does not require a diagram, not at all like all the other DNA polymerases.

Conclusion :

Therefore, these enzymes, Bacteriophage lambda exonuclease and terminal transferase are very much responsible for the formation of sticky ends in the plasmid.