which of the following has higher lemda max value
1-butene or 1,3-butadiane
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We had a blue colored solution whose lambda max should be around 750 nm. At low concentrated solution (100ug/ml), absorbance value upto 0.7, the lambda max was observed around 740 nm.
But in the concentrated solution, absorbance value greater than 1, lambda max shifts to 640 nm and further decreases with increasing absorbance value.
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