Question

Which of the following is a direct method of gene transfer?​

Answer 1

Answer:

Gene transfer technologies include techniques of electroporation, microinjection, macroinjection, biolistics or microprojectiles for DNA transfer (particle gun), liposome mediated gene transfer, calcium phosphate mediated DNA transfer, use of DAE-dextran to transfer gene, polycation-DMSO for DNA transfer, polyethylene glycol mediated transfection and gene transfer through peptides.

Explanation:

Answer 2

In the direct method gene is transferred without help of any vector.

Following are the some methods of direct gene transfer :-

1. Electroporation :- This method is normally used in the bacterias. In this electric shock is given which create pore in the cell.

2. Micro Injection :- This method is usually used in animal cells. Also it can be used in plants but is only possible after removal of the cell wall.

3. Gene Gun :- By this method first gene is converted into the bullet with the help of gold or tungsten. After this it is bombarded on the targeted cells with the help of the gene gun.

4. Chemical method :- PEG or poly ethylene glycol which works by altering the permeability of the membrane.