Question

Which of the following is not a characteristic of pBR322 vector ?

Answer 1

Explanation:

ANSWER

pBR322 was the first artificial cloning vector constructed in 1977 by Boliver and Rodriguez. It is widely used in gene cloning experiments. In pBR322 plasmid vector, p - denotes that it is a plasmid; BR - stands for Boliver and Rodriguez who constructed this plasmid; 322 - Is a number given to distinguish this plasmid from others developed in the same laboratory. E coli cloning vector pBR 322 has restriction sites {HindIII, EcoRI, BamHl, SaII, PvuII, PstI, ClaI), ori(origin of replication) and antibiotic resistance genes (amp

R

and tet

R

). rop codes for the proteins involved in the replication of the ] plasmid.

Answer 2

Explanation:

it was the first Artificial cloning vector constructed in 1977 bye Boliver and Rodriguez .

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