Question

Which of the following show a correct pair with respect to pBR322 ?

Answer 1

Answer:

It does not have restriction site for SaII

pBR322 was the first artificial cloning vector constructed in 1977 by Boliver and Rodriguez. It is widely used in gene cloning experiments. In pBR322 plasmid vector, p - denotes that it is a plasmid; BR - stands for Boliver and Rodriguez who constructed this plasmid; 322 - Is a number given to distinguish this plasmid from others developed in the same laboratory. E coli cloning vector pBR 322 has restriction sites {HindIII, EcoRI, BamHl, SaII, PvuII, PstI, ClaI), ori(origin of replication) and antibiotic resistance genes (amp

Answer 2

Answer:

pBR322 DNA is a commonly used plasmid cloning vector in E. coli. The molecule is a double - stranded circle 4,361 base pairs in length.