Which of the following statements are correct regarding the process of RNA interference ?
(i) RNAi has been used to prevent nematode infestation of tobacco plants .
(ii) RNAi takes place in all eukaryotic organisms as a method of cellular defense.
(iii) The method involves silencing of specific mRNA using a complementary dsDNA molecule that binds and prevents the translation of mRNA.
(iv) using retrovirus vectors , nematode–specific genes were introduced into the host plant .
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Correct option is
(iii) and (iv)
RNAI or RNA interference takes place in all eukaryotic organisms as a method of cellular defense. This method involves silencing of a specific mRNA. Using Agrobacterium vectors, nematode-specific genes are introduced of DNA was such that it produced both sense and anti-sense RNA in the host cells. These two RNAs being complementary to each other formed a dsRNA (double-stranded RNA) that initiated RNAI.
So, the correct answer is '(iii) and (iv)'.
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