Which of the following steps
are NOT performed in
Lyophilization
Answers
Answered by
1
Answer:
Explanation: In lyophilization or freeze-drying, a dense cell suspension is placed in small vials and frozen at -60 degree to -78 degree C.
Answered by
0
Mineral oil addition is not required during lyophilization.
Explanation:
In lyophilization, water is removed from the sample under low pressure and temperature.
It involves the conversion of ice crystals directly into vapor without the use of heat. This is called sublimation.
During the lyophilization of cell cultures, a dense suspension of cells is placed in vials and frozen (-60°C to -70°C).
The vial is connected to a high vacuum line. The ice crystals present undergo sublimation and thus the sample is lyophilized.
The addition of mineral oil to preserve the culture is not needed.
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