which part of the cells get more stain
Answers
Answer:
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Bacterial smears nd smpl stnd
Bctrl cells r uslly colrlss been or cytoplasm 4 the most part is it transpart .Since the bactrl r colrlss it is almost essntl 2 add a stain 2 bctria more visibl once stnd cell morphology can b obsrvd
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Answer:
NucSpot® 470 is cell membrane-impermeant green fluorescent DNA stain. It is virtually non-fluorescent in the absence of DNA, but fluoresces bright green upon DNA binding. While other green nucleic acid stains like TOTO®, TO-PRO®, or SYTOX® dyes stain both the nucleus and cytoplasm, NucSpot® 470 specifically stains the nucleus of fixed and permeabilized cells. It also can be used to selectively stain dead cells in living cultures. NucSpot® 470 has green fluorescence that can be imaged using standard settings for FITC. With excitation at 460 nm, it also is an excellent match for instruments with blue LED excitation sources.
NucSpot® Live 488 and NucSpot® Live 650 Nuclear Stains
NucSpot® Live Nuclear Stains specifically stain nuclei in live or fixed cells with no need for washing. NucSpot® Live 488 has green fluorescence (Ex/Em 500/515 nm), while NucSpot® Live 650 has far-red fluorescence (650/675 nm) for detection in the Cy®5 channel. Unlike Draq5™, NucSpot® Live 650 has low cytotoxicity and can be used for longer term imaging. NucSpot® Live 650 dye is also compatible with super-resolution imaging by SIM and STED.
RedDot™1 and RedDot™2 Far-Red Nuclear Stains
RedDot™1 and RedDot™2 are far-red nuclear counterstains for the Cy®5 channel. RedDot™1 is an alternative to DRAQ5™ that rapidly and specifically stains nuclei in live cells. It can be used for cell cycle analysis by flow cytometry. It also can be used for cell normalization for In Cell Western™. RedDot™1 shows cytotoxicity within a few hours of staining, so for long-term live cell imaging, we recommend NucSpot® Live 650.
RedDot™2 is membrane impermeant and can be used to selectively stain dead cells, or as a nuclear counterstain for fixed cells. RedDot™2 shows better nuclear specificity in fixed cells than DRAQ7™, which requires a blocking step for nuclear-specific counterstaining.
Live-or-Dye NucFix™ Red
Live-or-Dye NucFix™ Red is a unique, cell membrane impermeable dye that specifically stains the nuclei of dead cells. The dye is able to enter into dead cells that have compromised membrane integrity and covalently label the cell nucleus, allowing for clear differentiation of live and dead cells by either microscopy or flow cytometry. Unlike other commonly used nuclear stains such as propidium iodide or DRAQ7™, NucFix™ covalently attaches to DNA, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells.
Classic Blue Nuclear Stains
DAPI and Hoechst are widely used blue fluorescent nuclear counterstains. They are minor groove-binding DNA dyes that are minimally fluorescent in solution, but have strong fluorescence enhancement upon binding DNA.
DAPI is less membrane permeant than Hoechst, and is typically used to stain fixed cells at concentrations around 1 ug/mL. Antifade mounting medium with DAPI, like Biotium’s EverBrite™ Mounting Media, can be used for one-step mounting and counterstaining. Staining live cells with DAPI requires higher concentration (~10 ug/mL). We offer DAPI dilactate, a more water soluble DAPI salt, which can be used at higher concentrations.
Hoechst dyes are membrane-permeant and can be used for live or fixed cell staining and cell cycle analysis. Hoechst 33342 and Hoechst 33528 both are quenched by BrdU labeled DNA, and have been used in cell division studies. The two dyes are spectrally similar. Hoechst 33342 is slighly more cell permeable than Hoechst 33258, but both dyes are commonly used as nuclear stains for live or fixed cells at 1 ug/mL.
While Hoechst and DAPI show less cytotoxicity than intercalating DNA dyes, they bind DNA in living cells and are potentially hazardous. Biotium offers Hoechst 33342, Hoechst 33258, and DAPI as 10 mg/mL solutions in water, for greater convenience and safety compared to weighing out the powdered dyes.
DAPI and Hoechst undergo photoconversion by UV excitation to form green fluorescent dyes, which can lead to artifacts in multi-color imaging. See our Tech Tip Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst for more information.
Asymmetric Cyanine Dyes and Other Dead Cell Nuclear Stains
Biotium offers a selection of cyanine dyes for nucleic acids. Cyanine dyes have high affinity for DNA and RNA. Several of the membrane-impermeant dyes are useful dead cell stains. Oxazole Yellow (equivalent to YO-PRO®-1) is unique in that it selectively stains early apoptotic cells. Membrane-impermeant cyanine dyes also can be used as nuclear counterstains, but unlike RedDot™ or NucSpot® dyes, they stain both RNA and DNA, and so require RNase treatment for selective nuclear staining.
Explanation:
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