Question

Which protein helps in tethering of
DNA polymerase to the DNA?
1.SSB
2.RPA
3.MCM
4.PCNA​

Answer 1

(4)PCNA​elps in tethering of DNA polymerase to the DNA

Explanation:

1.Tethering of DNA in magnetic tweezers by coupling a multiply biotinylated handle on one extremity of the DNA to a streptavidin-coated magnetic bead,

2.and by binding a handle containing multiple digoxigenin molecules on the other extremity of the DNA to anti-digoxigenin IgG antibodies adsorbed on nitrocellulose.

3.PCNA is a central protein in the initiation and persistence of DNA replication, where it functions as a processivity factor for DNA polymerase δ [50]