Which quantity associated with light wave sets the limit of ability to distinguish very close objects?
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Principles of Microscopy
Brightfield microscopy
The microscope that is available to you for general use in this laboratory is a sophisticated optical instrument that can provide you with high-resolution images of a variety of specimens. Image quality is based largely on your ability to use the microscope properly. Below you will find some basic information that you have probably heard before, but information that is rarely presented in a thorough way.
- Resolution -
The magnification of small things is a necessary facet of biological research, but the fine detail in cells and in subcellular components requires that any imaging system be capable of providing spatial information across small distances. Resolution is defined as the ability to distinguish two very small and closely-spaced objects as separate entities. Resolution is best when the distance separating the two tiny objects is small. Resolution is determined by certain physical parameters that include the wavelength of light, and the light-gathering power of the objective and condenser lenses. A simple mathematical equation defines the smallest distance (dmin) separating the two very small objects:
Brightfield microscopy
The microscope that is available to you for general use in this laboratory is a sophisticated optical instrument that can provide you with high-resolution images of a variety of specimens. Image quality is based largely on your ability to use the microscope properly. Below you will find some basic information that you have probably heard before, but information that is rarely presented in a thorough way.
- Resolution -
The magnification of small things is a necessary facet of biological research, but the fine detail in cells and in subcellular components requires that any imaging system be capable of providing spatial information across small distances. Resolution is defined as the ability to distinguish two very small and closely-spaced objects as separate entities. Resolution is best when the distance separating the two tiny objects is small. Resolution is determined by certain physical parameters that include the wavelength of light, and the light-gathering power of the objective and condenser lenses. A simple mathematical equation defines the smallest distance (dmin) separating the two very small objects:
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