which techniques are used for detection of DNA?
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Answer:Southern blotting (a technique developed by E. M. Southern) is widely used for detection of specific genes in cellular DNA (Figure 3.29). The DNA to be analyzed is digested with a restriction endonuclease, and the digested DNA fragments are separated by gel electrophoresis.
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The methods used for the detection of DNA are:
- UV- Vis Spectrophotometric analysis.
- Fluorometric analysis.
- DNA Precipitation.
- DNA Gel electrophoresis.
- Polymerase chain reaction.
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