Question

Which vector is best insertional or replacement vector? ​

Answer 1

Replacement or Substitution vectors were developed in which a middle dispensable 'filler' fragmentis is discarded and replaced with the insert DNA.These vectors contain restriction sites flanking the non-essential region of phage. They can accommodate larger inserts than insertion vectors.

HOPE it helps to you!!

Answer 2

in insertional vector no phage DNA is removed and foreign DNA fragment are inserted.they have a single. recognition site for one or more restriction enzyme that cuts it only once.