Question

While clonning which of the two will preferred by the bitechnologist plasmid or bacteriophage?

Answer 1

Many types or kinds of cloning vectors are used for the transfer of gene of interest into the desired tissue or cell. In other words vectors are used as vehicle to carry gene of interest. Several types of vectors are being constructed in laboratory; each vector has got different molecular properties as well as cloning capacity. Some of the examples for vectors used in recombinant DNA technology are plasmids, phagemids, cosmids, shuttle vectors and much more. 

Plasmid as Cloning Vector: 

1. Plasmids are extra chromosomal circular double stranded DNA, present in bacterial cells. Plasmid are used as cloning vectors, include ori site or origin of replication, needed for replication in the bacterial cells. For an example E.coli plasmid vector, should have following features: 

a. Ori site for replication. 

b. Selectable marker genes, such as antibiotic resistance gene 

c. Unique restriction sites, so that the restriction enzymes can be used to cut the plasmid and DNA of interest can be inserted into the plasmid. 



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Answer 2

Bacteriophages

Bacteriophages have very high copy no.of their genome within bacterial cells whereas plasmids may have only one or two copies per cell