Question

Who modified Ziehl-Neelsen staining technique?
a. Louis Pasteur
b. Robert Koch
c. Ziehl-Neelsen
d. All the above

Answer 1

Thanks for the question!

It is definitely a very interesting question to solve and do some brainstorming.

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The answer is c. Ziehl-Neelsen

The Ziehl–Neelsen staining technique,( acid-fast staining ), was first described by two German doctors: the bacteriologist Franz Ziehl (1859–1926) and the pathologist Friedrich Neelsen (1854–1898). 
It is a bacteriological stain used in the identification procedure of acid-fast organisms, mainly Mycobacteria viz., Mycobacterium tuberculosis , the causal agent of the widespread tuberculosis (TB) disease.
The method requires controlled heating for its completion thoroughly. In the ‘hot’ ZN technique, the phenol-carbol fuchsin stain is heated so that the dye can easily penetrate the waxy mycobacterial cell wall.

The modified ZN technique was given by Ziehl-Neelsen themselves and is the ‘cold’ technique known as Kinyoun Method, in which the stains are not heated but the complete penetration is achieved by increasing concentration of basic fuchsin stain and phenol with the help of incorporation of a ‘wetting agent’ chemical.

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Hope it helps!

Answer 2

THANKS for your question ☺☺

OPTION C IS CORRECT ✔✔

THANKS

☺☺