Question

wht are the vectors for cloning genes in plants​

Answer 1

Plasmids are the most common vectors used in plant transformation.

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Answer 2

Answer:

$\underline \mathscr \purple{Question \: : - }$

wht are the vectors for cloning genes in plants_?

$\underline \mathscr \purple{Answer \: : - }$

Vectors for cloning genes in plants:

Ti plasmid of Agrobacterium tumefaciens: Agrobacterium tumefaciens, a pathogen of several dicot plants is able to deliver a piece of DNA known as T-DNA' to transform normal plant cells into a tumor and direct these tumor cells to produce the chemicals required by the pathogen. This bacterium invades plants at the site of wound, transforming them and nearby cells to form a turnor called crown gall.

When the bacterium contacts a damaged plant cell, it delivers a T-DNA (transferred DNA) fragment from plasmid into host cell that integrates at a random position in plant cells chromosome. The tumor inducing (Ti) plasmid of A. tumefaciens has now been modified into a cloning vector which is no more pathogenic to the plants but is still able to use the mechanism to deliver genes of our interest into a variety of plants as shown in figure below. Disarming of Ti plasmid involves replacement of harmful T-DNA of Ti plasmid of original A. tumefaciens by gene of interest.