Why acetocarmine is selected for chromosome staining?
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To stain plant chromosomes, a 1% solution of carmine in 45% acetic acid is used. Freshly fixed material is transferred into 1% acetocarmine for at least 30 min and then analyzed by the squash method. If the material was fixed for a longer time, it requires a longer staining time (up to several days) to reach good contrast. If the material is to be analyzed immediately, fix and stain the tissue in one step using the 1% acetocarmine solution.
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