Question

Why are bacterial cultures stored in 50% glycerol ag low temperatures?

Answer 1

Explanation:

➡️ You normally store bacterial stocks in about 10-15% glycerol final concentration. The temperature will affect longevity. They store fine at -20C for some months (but not years) whereas at -80C they are stable for decades. So it very much depends upon your requirements, whether this is short term or long term storage. Also if storing at -20 you should be very careful to not use a frost-free freezer because those cycle the temperature above 0 every day to remove the ice as mentioned by Matthew.

Answer 2

Answer:

$\huge\underline\red{\mathcal Hey\:Mate}$

When liquid bacteria cultures are added to a 50% glycerol solution, the glycerol infuses into the bacterial cells, making them structurally stable and allowing them to be stored safely

Hope it helps uh!!!