why are genes encoding antibiotic resistance considered useful selectable markers in E coli.explain with the help of one example.
Answers
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☺A selectable marker is a gene introduced into a cell, especially a bacterium or to cells in culture, that confers a trait suitable for artificial selection.
✔They are a type of reporter gene used in laboratory microbiology, molecular biology, and genetic engineering to indicate the success of a transfection or other procedure meant to introduce foreign DNA into a cell.
✔Selectable markers are often antibiotic resistance genes (An antibiotic resistance marker is a gene that produces a protein that provides cells expressing this protein with resistance to an antibiotic.).
✔Bacteria that have been subjected to a procedure to introduce foreign DNA are grown on a medium containing an antibiotic, and those bacterial colonies that can grow have successfully taken up and expressed the introduced genetic material.
✔Normally the genes encoding resistance to antibiotics such as ampicillin, chloroamphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli.
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The genes encoding antibiotic resistance considered useful selectable markers in E coli because, it has the ability to get transformed into cells which shows the resistant against the antibiotics.
Explanation:
For example, when the “recombinant DNA which has gene for resisting ampicillin is transferred into E.coli cells”, the host cells become transformed into ampicillin-resistant cells.
When the transformed cell is plated on agar plate containing ampicillin, only transformant will grow and non-transformant will not grow.