Why are only few drops of CuSO4
solution added during the biurettes
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why are only few drops of CuSO4 solution is added during the biuret test of proteins. The crucial component is the copper II ion (Cu2+) from the CuSO4. When peptide bonds are present in this alkaline solution, the Cu2+ions will form a coordination complex with 4 nitrogen atoms from peptide bonds.
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