Question

Why biological assays have to be carried out using biological buffers?

Answer 1

Important Buffers in Living Systems. A bufferis a chemical substance that helps maintain a relatively constant pH in a solution, even in the face of addition of acids or bases. Buffering is important in living systems as a means of maintaining a fairly constant internal environment, also known as homeostasis.

Answer 2

Answer:

The main purpose of a buffer in a biological system is to resist change in pH due to internal and external influences and maintain intracellular and extracellular pH within a narrow range.