Why cementite appears black in optical microscope and white in scanning electron microscope?
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The reason is pretty basic: color is a property of light (i.e., photons), and since electron microscopes use anelectron beam to image a specimen, there's no color information recorded. The area where electrons pass through the specimen appears white, and the area where electrons don't pass through appears black.
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The reason is pretty basic: color is a property of light (i.e., photons), and since electron microscopes use anelectron beam to image a specimen, there's no color information recorded. The area where electrons pass through the specimen appears white, and the area where electrons don't pass through appears black.
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You probably observed biological specimens through a microscope in your science lab back in high school. And if you are, or have been, a biology major, then it could safely be assumed that you’ve spent a considerable part of your academic life around microscopes.
You may already know that there are a few different kinds of microscopes, including simple microscopes, compound microscopes, stereo microscopes, electron microscopes and others. All of these are used in different settings: some of them are used in biology classes in high school, while other, more advanced microscopes (like electron microscope) are used in research laboratories where scientists tinker with and examine really, really small stuff, like the eye of a housefly.
If you’ve never seen what kinds of images microscopes produce)
It’s generally believed that images produced by microscopes lack color, i.e., they are black and white. And this is true, at least to some extent.
So, do all microscopes produce black-and-white images? If yes, what’s the reason behind that?
Microscopes can produce colored images
As mentioned earlier, microscopes come in different types and sizes, and some of them do produce colored images. Take light microscopes,
The magnified image that a light microscope produces contains color. In fact, if you use any ordinary optical microscope that magnifies up to 500x levels, then you’ll most likely see colors in the magnified image.
However, when you go beyond a certain level of magnification, colors start disappearing from (the magnified) images. This is because in order to see something under a microscope, the object must have a very thin cross-section. In addition to that, it also needs to be thin enough for light to pass through it (generally).
That being said, when you take a specimen so small and thin, there’s not really that much material that could add color to the light. Think of it this way: when you look at a drop of water, it appears perfectly colorless, but when you look at an ocean – which is basically a collection of trillions of colorless drops of water – it appears to be a magnificent blue.
You may already know that there are a few different kinds of microscopes, including simple microscopes, compound microscopes, stereo microscopes, electron microscopes and others. All of these are used in different settings: some of them are used in biology classes in high school, while other, more advanced microscopes (like electron microscope) are used in research laboratories where scientists tinker with and examine really, really small stuff, like the eye of a housefly.
If you’ve never seen what kinds of images microscopes produce)
It’s generally believed that images produced by microscopes lack color, i.e., they are black and white. And this is true, at least to some extent.
So, do all microscopes produce black-and-white images? If yes, what’s the reason behind that?
Microscopes can produce colored images
As mentioned earlier, microscopes come in different types and sizes, and some of them do produce colored images. Take light microscopes,
The magnified image that a light microscope produces contains color. In fact, if you use any ordinary optical microscope that magnifies up to 500x levels, then you’ll most likely see colors in the magnified image.
However, when you go beyond a certain level of magnification, colors start disappearing from (the magnified) images. This is because in order to see something under a microscope, the object must have a very thin cross-section. In addition to that, it also needs to be thin enough for light to pass through it (generally).
That being said, when you take a specimen so small and thin, there’s not really that much material that could add color to the light. Think of it this way: when you look at a drop of water, it appears perfectly colorless, but when you look at an ocean – which is basically a collection of trillions of colorless drops of water – it appears to be a magnificent blue.
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